BIOTECHNOLOGICAL METHOD OF ISOLATION OF ECDYSTEROIDS FROM SURFACE PART OF SILENE BRAHUICA

Abstract

The process of obtaining ecdysterone from the aerial part of Silene brahuica using 90% ethanol as an extractant was studied. First, optimal conditions were determined: raw material particle size — up to 5 mm, process temperature — 20-30 °C. A 5-fold extraction is proposed. Sequential liquid extraction is used for extract purification: after dilution with water, the extract is treated three times with extraction gasoline to remove hydrophobic impurities, followed by six times with butanol to remove hydrophilic impurities. For drying the silikin substance, microcrystalline cellulose in a 1:2 ratio was used on the dry residue of the aqueous solution. The developed technology for obtaining the adaptogenic and immunostimulatory drug “Silikin” was tested on pilot plants at the Institute of Chemistry of Plant Substances.