STUDYING THE TARGET ACTIVITY OF ASSOCIATIONS OF MICROORGANISMS CAPABLE OF REDUCING OIL VISCOSITY

Abstract

The article presents the results of studies of microorganisms capable of isolating target metabolites as candidate objects for the development of a microbiological method for increasing oil production from processed reservoirs. The aim of the study was to determine the target activity of associations of microorganisms capable of degreasing oil extracted from treated oil reservoir waters. Microorganisms in oil reservoir waters have a great biotechnological potential. They also produce metabolites (organic acids, biosurfactants, solvents, gases) for oil production. According to the results of a study of 3 associations of active acid-forming Bacillus sp. and Pseudomonas aeruginosa (D7X + D8; D8 + Cl1; D1X + D7 + D7X + D8 + Cl1) were selected. Thus, the maximum decrease in the pH of the nutrient medium (initial 7.0 units) was observed in the range of 4.1-4.2 units. The maximum oil emulsification index is shown in cultures D1X + D7; D7+D7X; D1X + D7 + D7X; D1X + D7 + D7X + D8 + Cl1 within 60.6-61%.

Two associations of microorganisms were isolated, consisting of two and five monocultures capable of reducing oil viscosity: Bacillus sp. D7X + Bacillus sp. D8; Bacillus sp D1X + P. aeruginosa D7 + Bacillus sp D7X + Bacillus sp D8 + Bacillus sp. Cl1 and their further study as promising microorganisms for the development of microbiological methods for enhanced oil recovery.